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MABN1559 Anti-ATBF1 Antibody, clone 5A1.1

Anti-ATBF1, clone 5A1.1, Cat. No. MABN1559, is a highly specific mouse monoclonal antibody that targets Zinc finger homeobox protein 3 and has been tested in Western Blotting.

Anti-ATBF1 Antibody, clone 5A1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ZFHX3, ATBF1 UniProt Number: Q15911
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MABN1559
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1559
      DescriptionAnti-ATBF1 Antibody, clone 5A1.1
      Alternate Names
      • Zinc finger homeobox protein 3
      • AT motif-binding factor 1
      • AT-binding transcription factor 1
      • Alpha-fetoprotein enhancer-binding protein
      • Zinc finger homeodomain protein 3
      • ZFH-3
      Background InformationZinc finger homeobox protein 3 (UniProt: Q15911; also known as AT motif-binding factor 1, AT-binding transcription factor 1, ATBF1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3) is encoded by the ZFHX3 (also known as ATBF1) gene (Gene ID: 463) in human. ATBF1 contains 23 C2H2-type zinc fingers and 4 homeobox DNA-binding domains. It is shown to translocate from the cytoplasm to the nucleus following TGF-beta stimulation. It acts as a transcriptional regulator that can act as an activator or a repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling, it can repress the transcription of AFP via its interaction with SMAD2/3. ATBF1 is shown to regulate myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. It is not found in normal gastric mucosa, but is found in gastric carcinoma cells and its expression is higher in ER-positive breast tumors than ER-negative breast tumors. It is shown to down-regulate the MUC5AC promoter in gastric cancer cells.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ATBF1, clone 5A1.1, Cat. No. MABN1559, is a highly specific mouse monoclonal antibody that targets Zinc finger homeobox protein 3 and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 87 amino acids from the C-terminal region of human Zinc finger homeobox protein 3.
      Clone5A1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5A1.1 recognizes Zinc finger homeobox protein 3 (ATBF1) in human cells. It targets an epitope with in 87 amino acids from the C-terminal region. This antibody recognizes isoform of 306 and 404 kDa.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZFHX3
      • ATBF1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~404 kDa observed; 404.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SHSY-5Y cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected ATBF1 in 10 µg of SHSY-5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1559 04054839250989