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MABE1789-25UG Anti-APC11 Antibody, clone CRQ1.1

Anti-APC11, clone CRQ1.1, Cat. No. MABE1789, is a highly specific mouse monoclonal antibody that targets Anaphase-promoting complex subunit 11 and has been tested in Western Blotting.

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MABE1789-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1789-25UG
      DescriptionAnti-APC11 Antibody, clone CRQ1.1
      Alternate Names
      • Anaphase-promoting complex subunit 11
      • Cyclosome subunit 11
      • Hepatocellular carcinoma-associated RING finger protein
      Background InformationAnaphase-promoting complex subunit 11 (UniProt: Q9NYG5; also known as APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein) is encoded by the ANAPC11 (also known as HSPC214) gene (Gene ID: 51529) in human. APC11 is one of the components of the anaphase-promoting complex (APC) that belongs to the RING-box family of proteins. APC is a large E3 ubiquitin ligase that responsible for targeting proteins for degradation during mitosis. The APC is assembled from 13 different proteins where in APC2 binds to APC11 forming the catalytic core that recruits the E2 ubiquitin conjugating enzyme. APC11 has with multiple zinc -binding sites with a RING-type zinc-finger domain (aa 34-77). It is expressed at high levels in the skeletal muscle and heart and in moderate levels in brain, kidney, and liver. Two isoforms of APC11 have been described that are produced by alternative splicing. (Ref.: Schreiber, A et al., (2011). Nature. 470(7333); 227-32).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-APC11, clone CRQ1.1, Cat. No. MABE1789, is a highly specific mouse monoclonal antibody that targets Anaphase-promoting complex subunit 11 and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected APC11 in Western Blotting applications (Sedgwick, G.G., et. al. (2013). EMBO J. 32(2):303-14).

      Western Blotting Analysis: A representative lot detected APC11 in APC11-Flag expressed in HeLa cells following tetracycline treatment.
      Biological Information
      ImmunogenA linear peptide corresponding to 9 amino acids from the C-terminal region of human Anaphase-promoting complex subunit 11 (APC11).
      EpitopeC-terminus
      CloneCRQ1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CRQ1.1 specifically detects human Anaphase-promoting complex subunit 11 (APC11). It targets an epitope with in 9 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey, and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANAPC11
      • HSPC214
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight9.84 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1789-25UG 04054839623592

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