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MABC677 Anti-AMACR Antibody, clone 2A10F3

Anti-AMACR Antibody, clone 2A10F3 is a highly specific mouse monoclonal antibody, that targets AMACR & has been tested in western blotting, IHC & ICC.

Anti-AMACR Antibody, clone 2A10F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RACE, AMACR UniProt Number: Q9UHK6
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MABC677
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC677
      DescriptionAnti-AMACR Antibody, clone 2A10F3
      Alternate Names
      • Alpha-methylacyl-CoA racemase
      • 2-methylacyl-CoA racemase
      Background InformationAlpha-methylacyl-CoA racemase (AMACR) is an enzyme responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their S-stereoisomers. It is a racemase (RACE), which means it will catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry. AMACR is localized in peroxisomes and mitochondria. Both increases and decreases of the levels of the enzyme have been linked with human diseases. In particular, reduction in AMACR activity or protein levels leads to an increase in bile acids which can result in neurological disorders and Refsum disease, a disorder that ultimately results in cerebellar degeneration and peripheral neuropathy. AMACR deficiency also can lead to peroxisome disorders that lead to the buildup of pristanic acid, DHCA and EHCA which can lead to mental impairment, confusion, leaning difficulties and liver damage. Increased levels of AMACR are associated with prostate cancer and AMACR is widely used as a cancer marker for prostate cancer. In cancer, the protein is called P504S. Finally, AMACR is responsible for the action of ibuprofen; AMACR will convert any R-enantiomer ibuprofen to S-enantiomer, which is the bioactive form that inhibits the activity of the cyclo-oxygenase enzymes and thus bring about the anti-inflammatory effect of the drug. EMD-Millipore’s Anti-AMACR mouse monoclonal antibody, clone 2A10F3, has been tested in western blot against cell lysates from the human prostate adenocarcinoma cell line LNCaP, as well as, Jurkat cells. The clone has also been tested in immunohistochemistry in human prostate, prostate cancer, and normal cerebellum paraffin embedded tissue sections, and in fluorescent immunocytochemistry against LNCaP cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • LNCap cell lysate
      PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-AMACR Antibody, clone 2A10F3 is a highly specific mouse monoclonal antibody, that targets AMACR & has been tested in western blotting, IHC & ICC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected AMACR in human prostate cancer and human kidney tissues.

      Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected AMACR in LNCaP cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human AMACR expressed in E. coli.
      Clone2A10F3
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RACE
      • AMACR
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~45 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms between ~22 kDa and ~44 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in LNCap cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AMACR in 10 µg of LNCap cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC677 04053252910029

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies