Millipore Sigma Vibrant Logo

110116 β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli

β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase

View This Product on Sigma-Aldrich
110116
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
110116-1UCN
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 1 u
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
      Catalogue Number110116
      Brand Family Calbiochem®
      SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
      References
      ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Product Information
      CAS number9012-33-3
      Activity≥ 35 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
      EC number3.2.1.30
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥ 80 units/mg protein
      Physicochemical Information
      ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      110116-1UCN 04055977208627

      Documentation

      β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      110116

      References

      Reference overview
      Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-April-2008 RFH
      SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
      DescriptionRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.0.
      CAS number9012-33-3
      EC number3.2.1.30
      ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
      Specific activity≥ 80 units/mg protein
      Activity≥ 35 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.