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70174 pT7Blue Blunt Vector (linearized) - Novagen

pT7Blue Blunt Vector (linearized) - Novagen: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
70174
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Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
70174-3 Fiala di plastica 20 rxn
70174-4 Fiala di plastica 40 rxn
Description
Overview

The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
Catalogue Number70174
Brand Family Novagen®
Features and benefits
  • No restriction enzymes or special primers
  • Compatible with any DNA polymerase
  • Independent of 3ʹ-dA addition or sequence context of DNA ends
  • Blue/white screening with seven different vectors, including pETBlue expression vectors
  • Simple protocol takes less than one hour* from PCR product to plating transformants

* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
References
References

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
70174-3 04055977258363
70174-4 04055977258370

Documentation

pT7Blue Blunt Vector (linearized) - Novagen Certificati d'Analisi

TitoloNumero di lotto
70174

Riferimenti bibliografici

Panoramica delle referenze

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

Citazioni

Titolo
  • Ekaterina Bogdanova, et al. (2008) Transcription regulation of the type II restriction-modification system AhdI. Nucleic Acids Research 36, 1429-1442.
  • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
  • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
  • Rahul Pal, et al. (2005) Evidence for multiple shared antigenic determinants within Ro60 and other lupus-related ribonucleoprotein autoantigens in human autoimmune responses. Journal of Immunology 175, 7669-7677.
  • Dana R. Rundle, et al. (2004) Myristoyl-CoA: protein N-myristoyltransferases: isoform identification and gene expression in retina. Molecular Vision 10, 177-185.
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    Protocolli per l'uso

    Titolo
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB183 Perfectly Blunt® Cloning Kits

    Mappa di un vettore

    Titolo
    TB017VM pT7Blue Vector Map

    Prodotti e applicazioni correlate

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    Categorie

    Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors