Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

71341 pRSFDuet™-1 DNA - Novagen

71341
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
71341-3 Fiala di plastica 10 μg
Description
OverviewpRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pRSF1030 replicon (also known as NTP1), lacI gene, and kanamycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and/or pCDFDuet™-1 for the coexpression of up to eight target proteins. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number71341
Brand Family Novagen®
Application Data
.
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
71341-3 04055977253283

Documentation

pRSFDuet™-1 DNA - Novagen MSDS

Titolo

Scheda di sicurezza (MSDS) 

pRSFDuet™-1 DNA - Novagen Certificati d'Analisi

TitoloNumero di lotto
71341

Citazioni

Titolo
  • Steven Raynard, Wendy Bussen and Patrick Sung. (2006) A double Holliday junction dissolvasome comprising BLM, topoisomerase III, and BLAP75. Journal of Biological Chemistry 281, 13861-13864.
  • Niraj H. Tolia and Leemor Joshua-Tor. (2006) Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55-64.
  • Protocolli per l'uso

    Titolo
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB340 Duet Vectors
    TB401 pRSF and pCDF Vectors

    Mappa di un vettore

    Titolo
    TB391 pRSFDuet™-1 Vector Map

    Sequenza del vettore

    Titolo
    pRSFDuet™-1 Vector Sequence