Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

69077 pET-30 Ek/LIC Vector Kit - Novagen

Novagen's pET-30 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the His-Tag and S-Tag coding sequences that are cleavable with enterokinase protease.

pET-30 Ek/LIC Vector Kit - Novagen: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
69077
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
69077-3 Fiala di plastica 20 rxn
Description
OverviewThe pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69077
Brand Family Novagen®
Application Data
References
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
69077-3 07790788055134

Documentation

pET-30 Ek/LIC Vector Kit - Novagen Certificati d'Analisi

TitoloNumero di lotto
69077

Brochure

Titolo
Radiance! Multisystem Protein Expression

Citazioni

Titolo
  • Rui He, et al. (2008) Metacaspase-8 modulates programmed cell death induced by ultraviolet light and H2O2 in Arabidopsis. Journal of Biological Chemistry 283, 774-783.
  • Steven G. Van Lanen, Shuangjun Lin and Ben Shen. (2008) Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. Procedings of the National Academy of Science 105, 494-499.
  • Anette Thysse Jonstrup, et al. (2007) The 1.4-Å crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme. Nucleic Acids Research 35, 3153-3164.
  • Mads Gravers Jeppesen, et al. (2005) Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex. Journal of Biological Chemistry 280, 5071-5081.
  • Francisco J. Sandoval and Sanja Roje. (2005) An FMN hydrolase is fused to a riboflavin kinase homolog in plants. Journal of Biological Chemistry 280, 38337-38345.
  • Jongmin Lee, et al. (2004) Transglutaminase 2 induces nuclear factor-κB activation via a novel pathway in BV-2 microglia. Journal of Biological Chemistry 279, 53725-53735.
  • Beata Zalewska, et al. (2003) Chimeric Dr fimbriae with a herpes simplex virus type 1 epitope as a model for a recombinant vaccine. Infection and Immunity 71, 5505-5513.
    		•

    Protocolli per l'uso

    Titolo
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual
    TB163 Ek/LIC Cloning Kits
    TB184 Xa/LIC Cloning Kits

    Mappa di un vettore

    Titolo
    TB162VM pET-30 Ek/LIC Vector Map

    Sequenza del vettore

    Titolo
    pET-30 Ek/LIC Vector Sequence