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70088 pBAC™-3 DNA - Novagen

70088
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
70088-3 Fiala di plastica 10 μg
Description
OverviewpBAC™-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.





This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70088
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
70088-3 04055977258707

Documentation

pBAC™-3 DNA - Novagen MSDS

Titolo

Scheda di sicurezza (MSDS) 

pBAC™-3 DNA - Novagen Certificati d'Analisi

TitoloNumero di lotto
70088

Brochure

Titolo
Insect Cell Expression

Citazioni

Titolo
  • Hyesook Yoon, et al. (2007) Activation profiles and regulatory cascades of the human kallikrein-related peptidases. Journal of Biological Chemistry 282, 31852-31864.
  • Mappa di un vettore

    Titolo
    TB217 pBAC™-3 Vector Map

    Sequenza del vettore

    Titolo
    pBAC-3 Sequence