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654205 TNF-α, Human, Recombinant, E. coli

TNF-α, Human, Recombinant, E. coli: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α

654205
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
654205-10UG Fiala di plastica 10 μg
Description
OverviewRecombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
Catalogue Number654205
Brand Family Calbiochem®
SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
References
ReferencesMerck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
Product Information
CAS number94948-59-1
FormLyophilized
FormulationCarrier-free
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSMT6435000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
654205-10UG 04055977184594

Documentation

TNF-α, Human, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

TNF-α, Human, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
654205

Riferimenti bibliografici

Panoramica delle referenze
Merck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-January-2009 JSW
SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
DescriptionRecombinant, human tumor necrosis factor-α expressed in E. coli. Activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein (SAP) kinase.
FormLyophilized
FormulationCarrier-free
CAS number94948-59-1
RTECSMT6435000
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
SolubilityReconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Toxicity Standard Handling
ReferencesMerck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.