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69025 T7•Tag® Affinity Purification Kit

69025
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
69025-3 Bottiglia di vetro 1 kit
Description
OverviewThe T7•Tag® Affinity Purification Kit is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (e.g., the 11 amino terminal aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Neutralization Buffer is included to limit protein exposure to low pH. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity.
Catalogue Number69025
Brand Family Novagen®
References
Product Information
Components
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
69025-3 04055977274707

Documentation

T7•Tag® Affinity Purification Kit MSDS

Titolo

Scheda di sicurezza (MSDS) 

T7•Tag® Affinity Purification Kit Certificati d'Analisi

TitoloNumero di lotto
69025

Citazioni

Titolo
  • Hayakawa, H., et al. 2007. Soluble Journal of Biological Chemistry 282, 26369.
  • Chang-Sik Oh and Steven V. Beer. 2007. Plant Physiology 145, 426.
  • Sukumar, N., et al. 2007. Journal of Bacteriology 189, 3695.
  • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
  • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
  • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. 2003. The Cell 113, 127.
  • Protocolli per l'uso

    Titolo
    TB055 pET System Manual
    TB125 T7•Tag® Affinity Purification Kit