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GF240 Sema3A Recombinant Protein

Sema3A Recombinant Protein: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
UniProt Number: Q14563
GF240
10ug  
Purchase on Sigma-Aldrich

Panoramica

Description
Catalogue NumberGF240
DescriptionSema3A Recombinant Protein
Background InformationSemaphorins are a large family of well conserved proteins that participate in a variety of cell signaling functions. However, they are best known for their role in development and regeneration within the nervous system. These affects have been shown to be primarily mediated by the class 3 semaporins (Sema3). In particular, the Sema3A family member acts as both a chemorepellant for axonal growth and a chemoattractant for dendritic processes1,2.

Here we provide recombinant Sema3A protein to facilitate researchers studying neurite outgrowth. The protein produced is an Fc-chimera containing the highly conserved semphorin domain, the Ig-like domain, and the highly positively charged C-terminal region (without the 20 amino acids at the C-terminus)3.
Product Information
PresentationProtein A affinity purified recombinant Sema3A protein in PBS buffer.
Quality LevelMQ100
Biological Information
Concentration0.25 mg/mL
PurityThe purity of the Sema3A protein was also determined by SDS-PAGE. Purified protein (2µg) was run using non-denaturing conditions and then coomassie blue stained. The protein detected was approximately 125kD in size.
Species Reactivity
  • Chicken
  • Human
  • Mouse
  • Rat
Gene Symbol
  • Hsema-I
  • Hsema-III
  • SEMA1
  • SEMAD
  • SEMAIII
  • SEMAL
  • SemD
  • coll-1
  • semaphorin-like
Purification Method40µl of 0.25mg/ml recombinant protein.
UniProt Number
UniProt SummaryFUNCTION: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1 By similarity.

SUBCELLULAR LOCATION: SecretedBy similarity.

DOMAIN: Strong binding to neuropilin is mediated by the carboxy third of the protein.

SEQUENCE SIMILARITIES: Belongs to the semaphorin family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 PSI domain.

Contains 1 Sema domain.
Product Usage Statements
Quality AssuranceGrowth cone collapse testing was carried out to verify recombinant Sema3A protein induces collapse. Briefly, dorsal root ganglion cells were plated and allowed to grow overnight in differentiation media. The next day the media was replaced with differentiation media or differentiation media with 1µg/ml of Sema3A protein. The cells were then incubated for 30 minutes, fixed, and stained with FITC conjugated antibodies specific for phalloidin (Part No. 90228). Representative images of each assay condition are shown below:
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C or at -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size10ug
Global Trade Item Number
Numero di catalogo GTIN
GF240 04053252474934