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03-0055-00 SMC™ Human Interleukin 8 (IL-8)
IMMUNOASSAY KIT

The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ IL-8 Immunoassay Kit unparalleled performance in the quantification of IL-8 in human EDTA plasma and serum.

SMC™ Human Interleukin 8 (IL-8)
IMMUNOASSAY KIT: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors
03-0055-00
96 well assay  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Analytes AvailableSpecies ReactivityKey Applications
IL-8 HSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0055-00
Trade Name
  • SMC®
DescriptionSMC™ Human Interleukin 8 (IL-8)
IMMUNOASSAY KIT
OverviewThe SMC™ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin-8 (IL-8) is a CXC chemokine synthesized by many cell types, including macrophages, epithelial cells and endothelial cells in response to a pathogen or inflammatory stimulus. IL-8 functionendothelials as a chemotactic factor that attracts neutrophils, basophils, and T-cells. IL-8 is one of the major mediators of the inflammatory response and of innate immunity. Its function is to recruit neutrophils to phagocytose the foreign antigen. IL-8 is also a potent angiogenic factor that induces neutrophils to leave the bloodstream and enter into the surrounding tissue. IL-8 has been shown to have roles in the pathological angiogenesis that is associated with solid tumors, diabetic retinopathy and rheumatoid arthritis (RA).
References
Product Information
Components
  • Human IL-8 Coated Beads
  • Standard Diluent
  • Human IL-8 Detection Antibody
  • Assay Buffer
  • Human IL-8 Standard
  • SMC™ Human IL-8 Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer D
HS Code3002 15 90
Precision, %
  • Low-end CV% Range: 6 - 18%
  • Low-end CV% Average: 12%
Quality LevelMQ200
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ IL-8 Immunoassay Kit unparalleled performance in the quantification of IL-8 in human EDTA plasma and serum.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • IL-8
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
03-0055-00 04055977318043