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HTS180RTA Ready-to-Assay™ NPBW1 Neuropeptide B/W Receptor Frozen Cells

Human NPBW1 / GPR7 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-4 Entrez Gene Number: NM_005285
HTS180RTA
1 kit  
Purchase on Sigma-Aldrich

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Tabella delle specifiche principali

SpeciesHost CellsProtein Target
Human Chem-4NPBW1 / GPR7
Description
Catalogue NumberHTS180RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ NPBW1 Neuropeptide B/W Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Neuropeptide B (NPB) and neuropeptide W (NPW) are members of a recently identified neuropeptide family that are ligands for two highly similar receptors, NPBW1 (GPR7) and NPBW2 (GPR8), both of which couple to Gi/o to inhibit intracellular cAMP production. Highest expression of NPBW1 mRNA and protein was identified in the amygdala and hypothalamic nuclei. Physiological studies demonstrate that intracerebroventricular infusion of NPBW1 ligands modulates feeding behaviour, nociception, and release of corticosterone, prolactin and growth hormone (Singh and Davenport, 2006). NPBW1 knock out male mice have shown mild adult-onset obesity and decreased locomotor activity. They become progressively hyperglycaemic and hyperinsulinaemic (Ishii et al., 2003). Millipore’s cloned human NPBW1-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant NPBW1 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at NPBW1.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman NPBW1 / GPR7 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-4
Protein TargetNPBW1 / GPR7
Target Sub-FamilyNeuropeptide B/W
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
HTS180RTA 04053252555367

Documentation

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Categorie

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells