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HTS026RTA Ready-to-Assay™ MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells

Human MCHR2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: AY562946
HTS026RTA
1 kit  
Purchase on Sigma-Aldrich

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Tabella delle specifiche principali

SpeciesHost CellsProtein Target
Human Chem-1MCHR2
Description
Catalogue NumberHTS026RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Melanin-concentrating hormone (MCH) is a cyclic, 19 amino acid peptide produced in the lateral hypothalamus and other regions of the mammalian brain (Bittencourt et al., 1992). When administered intracerebrally or overexpressed transgenically in rodents, MCH stimulates food intake and weight gain (Ludwig et al., 2001). Two G protein-coupled receptors for MCH, MCH1 and MCH2, have been identified in humans, although rodents appear only to have MCH1 (Tan et al., 2002). Both MCH1 and MCH2 are expressed primarily in brain (An et al., 2001). MCH1 has been demonstrated by genetic and pharmacological methods in rodent models to mediate obesity caused by a high fat diet or leptin deficiency (Borowsky et al., 2002; Segal-Lieberman, et al., 2003; Marsh et al., 2002). However, MCH2 might play a similar role in the species that express it. Millipore’s cloned human MCH2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MCH2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MCH2.
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman MCHR2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetMCHR2
Target Sub-FamilyMelanin-concentrating hormone
Cell Line Type
  • GPCR Cell Lines
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size1 kit
Global Trade Item Number
Numero di catalogo GTIN
HTS026RTA 04053252377877