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EZRMFGF21-26K Rat / Mouse FGF-21 ELISA Kit

EZRMFGF21-26K
96-well strip plate  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
FGF-21 R, MELISAColorimetric Fluorescent
Description
Catalogue NumberEZRMFGF21-26K
DescriptionRat / Mouse FGF-21 ELISA Kit
Background InformationRecent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. No significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.

Although considerable progress has been made in the understanding of the pathobiology of type 2 diabetes, as well in the development of new compounds for the treatment of diabetic patients, this disease remains a major global health concern. Consequently, Millipore is pleased to offer the Rat/Mouse FGF-21 ELISA kit for the quantification of FGF-21 in rat and mouse serum and plasma samples. The insulin-independent activities of FGF-21, as well as the absence of the weight gain effects of insulin, thiazolidinediones and other anti-diabetic drugs, distinguish it from current insulin-sensitizing drugs, making it a promising therapeutic.
References
Product Information
Detection methodColorimetric Fluorescent
HS Code3002 15 90
Precision, %
  • Intra-assay: < 10%
  • Inter-assay: < 8 %
Quality LevelMQ200
Applications
ApplicationThis Rat / Mouse FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.
Key Applications
  • ELISA
Application NotesThis is a 3-hour assay requiring 20 μL (in duplicate) of rat sample or 10µl (in duplicate) of mouse sample.
Biological Information
SpecificityCross-reactivity to Human FGF-21: 39%

No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.
Species Reactivity
  • Rat
  • Mouse
Analytes Available
  • FGF-21
Accuracy
  • Spike Recovery: 85 – 102 %
Physicochemical Information
Linearity of DilutionPlease contact Technical Service for this information
Standard Curve Range
  • 50 – 12000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
EZRMFGF21-26K 04053252407482

Documentation

Protocols

Title
Protocol- EZRMFGF21-26K
Protocol: Rat/Mouse Fibroblast Growth Factor-21 (FGF-21)

Rat / Mouse FGF-21 ELISA Kit MSDS

Titolo

Scheda di sicurezza (MSDS) 

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Induction of ketosis in rats fed low-carbohydrate, high fat diets depends on the relative abundance of dietary fat and protein.
Bielohuby, Maximilian, et al.
American journal of physiology. Endocrinology and metabolism, (2010)  2009

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Informazioni tecniche

Titolo
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- RMFGF-112 and RMFGF-212
Quality Control Ranges: RAT/MOUSE FGF-21 ELISA