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553510 RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem

A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis.

RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: 3-((Benzylamino)sulfonyl)-4-bromo-N-(4-bromophenyl)-benzamide, RS-1

553510
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Panoramica

Replacement Information

Tabella delle specifiche principali

CAS #Empirical Formula
312756-74-4C₂₀H₁₆Br₂N₂O₃S

Products

Numero di catalogoConfezionamento Qtà/conf
553510-10MG Fiala di plastica 10 mg
Description
OverviewA cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 & TALEN-mediated knock-in efficiencies.
Catalogue Number553510
Brand Family Calbiochem®
Synonyms3-((Benzylamino)sulfonyl)-4-bromo-N-(4-bromophenyl)-benzamide, RS-1
References
ReferencesSong, J., et al. 2016. Nat. Comm., In Press.
Jayathilaka, K., et al. 2008. Proc. Natl. Acad. Sci. USA. 105, 15848.
Product Information
CAS number312756-74-4
FormLight beige solid
Hill FormulaC₂₀H₁₆Br₂N₂O₃S
Chemical formulaC₂₀H₁₆Br₂N₂O₃S
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
553510-10MG 04055977193695

Documentation

RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
553510

Riferimenti bibliografici

Panoramica delle referenze
Song, J., et al. 2016. Nat. Comm., In Press.
Jayathilaka, K., et al. 2008. Proc. Natl. Acad. Sci. USA. 105, 15848.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-February-2016 MJ
Synonyms3-((Benzylamino)sulfonyl)-4-bromo-N-(4-bromophenyl)-benzamide, RS-1
DescriptionA cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 & TALEN-mediated knock-in efficiencies.
FormLight beige solid
Intert gas (Yes/No) Packaged under inert gas
CAS number312756-74-4
Chemical formulaC₂₀H₁₆Br₂N₂O₃S
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (100 mg/ml) or Ethanol (10 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up 3 months at -20°C.
Toxicity Standard Handling
ReferencesSong, J., et al. 2016. Nat. Comm., In Press.
Jayathilaka, K., et al. 2008. Proc. Natl. Acad. Sci. USA. 105, 15848.