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69078 Perfect Protein™ HRP Western Blot Kit

69078
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
69078-3 Fiala di plastica 25 blot
Description
OverviewThe Perfect Protein™ HRP Western Blot Kit includes an HRP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.


A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.
Catalogue Number69078
Brand Family Novagen®
Features and benefits• Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection.
• S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
• Can be used with colorimetric and chemiluminescent AP or HRP substrates.
• Recombinant, unmodified markers give sharp, accurately sized bands.
• Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
• Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
References
Product Information
Components
FormLiquid
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
69078-3 04055977259551

Documentation

Perfect Protein™ HRP Western Blot Kit MSDS

Titolo

Scheda di sicurezza (MSDS) 

Perfect Protein™ HRP Western Blot Kit Certificati d'Analisi

TitoloNumero di lotto
69078

Citazioni

Titolo
  • Yulin Jia, et al. (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. European Molecular Biology Organization Journal 19, 4004-4014.
  • Protocolli per l'uso

    Titolo
    TB102 Perfect Protein™ Western Blot Kits
    TB136 S-protein HRP Conjugate