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53702 PRONASE® Protease, Streptomyces griseus

53702
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
53702-10KU Bottiglia di vetro 10 ku
53702-250KU Bottiglia di vetro 250 ku
53702-25KU Bottiglia di vetro 25 ku
53702-500KU Bottiglia di vetro 500 ku
53702-50KU Bottiglia di vetro 50 ku
Description
OverviewA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number53702
Brand Family Calbiochem®
References
ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.
Product Information
CAS number9036-06-0
Activity≥45,000 proteolytic units/g dry weight
Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
FormLyophilized
FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSUK9595000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
53702-10KU 04055977195835
53702-250KU 04055977195859
53702-25KU 04055977195842
53702-500KU 07790788051259
53702-50KU 04055977195866

Documentation

PRONASE® Protease, Streptomyces griseus MSDS

Titolo

Scheda di sicurezza (MSDS) 

PRONASE® Protease, Streptomyces griseus Certificati d'Analisi

TitoloNumero di lotto
53702

Riferimenti bibliografici

Panoramica delle referenze
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

Brochure

Titolo
Bulk Product Guide
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
DescriptionA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
FormLyophilized
FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
CAS number9036-06-0
RTECSUK9595000
Activity≥45,000 proteolytic units/g dry weight
Unit definitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
SolubilityH₂O (10 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
Toxicity Harmful
ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.