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482777 Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli

482777
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
482777-50U Fiala di plastica 50 u
Description
OverviewRecombinant, mouse inducible NOS expression in E. coli.
Catalogue Number482777
Brand Family Calbiochem®
SynonymsInducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant
References
ReferencesStevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1.0 nmol nitric oxide per min at 37°C, pH 7.4.
FormLiquid
FormulationIn 50 mM HEPES, 10% glycerol, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥2.9 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-36/37/38

Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
482777-50U 04055977200645

Documentation

Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
482777

Riferimenti bibliografici

Panoramica delle referenze
Stevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-January-2011 JSW
SynonymsInducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant
DescriptionRecombinant, mouse inducible NOS expression in E. coli.
FormLiquid
FormulationIn 50 mM HEPES, 10% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions50 mM HEPES, 1 mM arginine, 1 mM magnesium acetate, 0.1 mM NADPH, 5 µM oxyhemoglobin, 12 µM tetrahydrobiopterin, 170 µM DTT, pH 7.4.
Specific activity≥2.9 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will produce 1.0 nmol nitric oxide per min at 37°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Harmful
ReferencesStevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.