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420200 JC-1 - CAS 47729-63-5 - Calbiochem

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.

JC-1 - CAS 47729-63-5 - Calbiochem: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: 5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide

420200
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

CAS #Empirical Formula
47729-63-5C₂₅H₂₇Cl₄IN₄

Products

Numero di catalogoConfezionamento Qtà/conf
420200-5MG Fiala di plastica 5 mg
Description
OverviewA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨm in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
Catalogue Number420200
Brand Family Calbiochem®
Synonyms5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
References
ReferencesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
Product Information
CAS number47729-63-5
ATP CompetitiveN
FormRed-orange solid
Hill FormulaC₂₅H₂₇Cl₄IN₄
Chemical formulaC₂₅H₂₇Cl₄IN₄
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetUsed as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
420200-5MG 04055977210743

Documentation

JC-1 - CAS 47729-63-5 - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

JC-1 - CAS 47729-63-5 - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
420200

Riferimenti bibliografici

Panoramica delle referenze
Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.

Brochure

Titolo
Caspases and other Apoptosis Related Tools Brochure
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-July-2008 RFH
Synonyms5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
DescriptionA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨm in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
FormRed-orange solid
Intert gas (Yes/No) Packaged under inert gas
CAS number47729-63-5
Chemical formulaC₂₅H₂₇Cl₄IN₄
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
Toxicity Irritant
ReferencesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.