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SCC190 J14 Human SLP-76-Deficient Jurkat Cell Line

J14 cells are a unique cell line that has proven particularly valuable for elucidating cellular signaling mechanisms and interaction network biology.

SCC190
≥1X10⁶ cells/vial   
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Replacement Information
Description
Catalogue NumberSCC190
DescriptionJ14 Human SLP-76-Deficient Jurkat Cell Line
OverviewJ14 cells are a unique Jurkat-derived human T cell line that lacks expression of SLP-76 (SH2 domain–containing leukocyte protein of 76 kilodaltons), an adaptor protein and PTK substrate expressed in hematopoietic cells (2). SLP-76 together with the scaffolding protein LAT act as a hub for various signaling effectors (1). Except SLP-76, the expression profile of J14 cells encompasses all necessary components of downstream pathways including those required for IL-2 cytokine induction (3), making the J14 cell line ideal for mutational analysis and introduction of reporter constructs.

Source:
J14 cells are a clonal derivative of Jurkat, a T cell line derived from bone marrow of a 14-year old male (4). Jurkat cells were transfected with an episomal vector and selected for hygromycin resistance. The transgene was subsequently lost via non-selective growth (2).
Alternate Names
  • Jurkat J14
  • J14-v-29
Background InformationT-cell-receptor signaling has a critical role in adaptive immune responses and has been extensively studied as a general model for complex cellular responses (1). T-cell-receptor signaling to downstream pathways is mediated by non-receptor protein tyrosine kinases (PTKs), which interact with substrate proteins in a complex network.

References:
1. J Cell Sci. 2009; 122(Pt 9): 1269-1273.
2. J Biol Chem. 1995; 270(13): 7029-7032.
3. Science. 1998; 281(5375): 413-416.
4. Int J Cancer. 1977; 19(5): 621-626.
References
Product Information
Applications
ApplicationJ14 cells are a unique cell line that has proven particularly valuable for elucidating cellular signaling mechanisms and interaction network biology.
Key Applications
  • Cell Culture
Application NotesThis product is sold solely for research use per the terms of the “Restricted Use Agreement” which govern its use as detailed in the product Data Sheet or Certificate of Analysis. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Blood Cell
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
SCC190 04054839752834