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IPFL00010 Membrana Immobilon-FL, PVDF, 0,45 µm, rotolo 26,5 cm x 3,75 m

Western blotting, dot/slot blotting

Membrana Immobilon-FL, PVDF, 0,45 µm, rotolo 26,5 cm x 3,75 m: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Applicazioni fondamentali: Western Blotting, Dot Blot
IPFL00010
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Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Applicazioni fondamentali
WB, DB
Descrizione
Numero di catalogoIPFL00010
Nome commerciale
  • Immobilon
DescrizioneMembrana Immobilon-FL, PVDF, 0,45 µm, rotolo 26,5 cm x 3,75 m
Informazioni di baseOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
Riferimenti bibliografici
Informazioni sul prodotto
Metodo di rilevazioneFluorescente Chemifluorescente Cromogenico Chemiluminescenza 125I Fluorescente
Coloranti compatibili
  • Tutti i coloranti fluorescenti
  • Coomassie brilliant blue
  • Amido black
  • Rosso Ponceau-S
  • CPTS
  • Sypro® Ruby
Codice del filtroIPFL
Colore del filtroBianco
Quality LevelMQ400
Applicazioni
ApplicazioneWestern blotting, dot/slot blotting
Applicazioni fondamentali
  • Western Blotting
  • Dot Blot
Informazioni biologiche
Adsorbimento di BSA205 µg/cm²
Adsorbimento di IgG di capra300 µg/cm²
Adsorbimento di insulina155 µg/cm²
BagnabilitàIdrofobo
Informazioni chimico-fisiche
Dimensioni dei pori0.45 µm
Dimensioni
Superficie del filtroPiana
Dimensioni del filtro27 cm x 3.75 m
Informazioni sui materiali
Chimica
  • PVDF idrofobo
Informazioni tossicologiche
Informazioni sulla sicurezza secondo il GHS
Informazioni per la sicurezza
Indicazioni sull'impiego del prodotto
Informazioni per lo stoccaggio e la spedizione
Informazioni sul confezionamento
Quantità1
Informazioni per il trasporto
Informazioni supplementari
Specifiche
Global Trade Item Number
Numero di catalogo GTIN
IPFL00010 04053252669439

Documentation

Membrana Immobilon-FL, PVDF, 0,45 µm, rotolo 26,5 cm x 3,75 m MSDS

Titolo

Scheda di sicurezza (MSDS) 

Brochure

Titolo
SNAP i.d. 2.0 System Brochure
Western Blotting Tools

Informazioni tecniche

Titolo
Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
Protein Blotting Handbook: 6th Edition

Posters

Titolo
Low Background Membrane for Fluorescent Protein Detection in Western Blotting

Manuali d'uso

Titolo
Immobilon® Block Noise Cancelling Reagents
Immobilon®-FL Transfer Membrane User Guide
User Guide - Immobilon® FL Transfer Membrane