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FC010-5MG Human Plasma Fibronectin Purified Protein

Human Plasma Fibronectin Purified Protein: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Key Applications: Cell Culture
FC010-5MG
5 mg  5 x 1 mg
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Key Applications
CULT
Description
Catalogue NumberFC010-5MG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHuman Plasma Fibronectin Purified Protein
OverviewHuman fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
References
Product Information
PresentationLiquid in 50 mM tris-buffered saline (TBS), pH 7.5, containing no preservatives.
Quality LevelMQ100
Applications
Key Applications
  • Cell Culture
Application NotesImmunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3. Introduce the proper CO2 atmosphere, if required.

4. Add the calculated amount of HFN to each culture vessel.

5. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6. Remove residual balanced salt solution before proceeding with standard cell culture procedures
Biological Information
Concentration1 mg/mL
PurityApproximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
SourceHuman donor plasma.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Gene Symbol
  • FN1
  • DKFZp686O13149
  • DKFZp686H0342
  • DKFZp686I1370
  • MSF
  • FN
  • CIG
  • DKFZp686F10164
  • LETS
  • FINC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
Packaging Information
Material Size5 mg
Material Package5 x 1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
FC010-5MG 04053252331183

Documentation

Human Plasma Fibronectin Purified Protein MSDS

Titolo

Scheda di sicurezza (MSDS) 

Human Plasma Fibronectin Purified Protein Certificati d'Analisi

TitoloNumero di lotto
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2147051 2147051
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2382597 2382597
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2445845 2445845
HUMAN PLASMA FIBRONECTIN - 3172081 3172081
HUMAN PLASMA FIBRONECTIN - 3179932 3179932
HUMAN PLASMA FIBRONECTIN - 3202373 3202373
HUMAN PLASMA FIBRONECTIN - 3221086 3221086
HUMAN PLASMA FIBRONECTIN - 3256639 3256639
HUMAN PLASMA FIBRONECTIN - 3279313 3279313
HUMAN PLASMA FIBRONECTIN - 3308854 3308854
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Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Comparative studies on amniotic fluid and plasma fibronectins
Ruoslahti E. et al.
Biochem. J, 193:295-299 (1981)  1981

7305927 7305927

Brochure

Titolo
Human Stem Cell Systems

Scheda tecnica

Titolo
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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