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AP181P Goat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed

AP181P
500 µL  
Purchase on Sigma-Aldrich

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Panoramica

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Description
Catalogue NumberAP181P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed
Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
FormatHRP
PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.
Quality LevelMQ100
Applications
ApplicationThis Goat anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Mouse IgG.
Key Applications
  • ELISA
  • Western Blotting
Application NotesEIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.
Biological Information
Concentration0.8 mg/ml
HostGoat
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Packaging Information
Material Size500 µL
Global Trade Item Number
Numero di catalogo GTIN
AP181P 04053252675713