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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

F1904 Sigma-AldrichFibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

MSDS
Cert. d'Analisi
Riferimenti bibliografici
Scheda tecnica

Key Applications: Cell Culture

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Panoramica

Replacement Information

Tabella delle specifiche principali

Key Applications
CULT

Description
Catalogue Number	F1904
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified
Overview	PROPERTIES: Contains the cell attachment region of the fibronectin molecule.

References

Product Information
HS Code	3502 90 90
Presentation	Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.
Quality Level	MQ100

Applications
Key Applications	Cell Culture
Application Notes	F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

Biological Information
Concentration	1 mg/mL
Purity	One major band at 120kDa in SDS-PAGE
Source	Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.
Entrez Gene Number	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
Entrez Gene Summary	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Gene Symbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProt Number	P02751
UniProt Summary	FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
Stem Cell Type	Human Embryonic Stem Cells Mesenchymal Stem Cells Neural Stem Cells Hematopoietic Stem Cells Epithelial Cells Pancreatic Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain material at -20°C for 3 months or -70°C for six months.

Packaging Information
Material Size	500 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numero di catalogo	GTIN
F1904	04053252279539

Documentation

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified MSDS

Titolo
Scheda di sicurezza (MSDS)

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified Certificati d'Analisi

Titolo	Numero di lotto
PURIFIED HUMAN FIBRONECTIN	2470386
PURIFIED HUMAN FIBRONECTIN ALPHA-CHYMOTRYPTIC FRAGMENT 120K (cell attachment) - 2387763	2387763
PURIFIED HUMAN FIBRONECTIN - 3821672	3821672
PURIFIED HUMAN FIBRONECTIN -2595768	2595768
PURIFIED HUMAN FIBRONECTIN -2605443	2605443
PURIFIED HUMAN FIBRONECTIN -2668113	2668113
PURIFIED HUMAN FIBRONECTIN -2736695	2736695
PURIFIED HUMAN FIBRONECTIN ALPHA-CHYMOTRYPTIC FRAGMENT 120K (cell attachment)	2933271
PURIFIED HUMAN FIBRONECTIN ALPHA-CHYMOTRYPTIC FRAGMENT 120K (cell attachment)	3135961
PURIFIED HUMAN FIBRONECTIN ALPHA-CHYMOTRYPTIC FRAGMENT 120K (cell attachment)	2903951

Riferimenti bibliografici

Panoramica dei riferimenti bibliografici	Codice d'identificazione nel Pub Med
Fibronectin: purification, immunochemical properties, and biological activities. Ruoslahti, E, et al. Meth. Enzymol., 82 Pt A: 803-31 (1982) 1981	7078457
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule. Pierschbacher, M D, et al. Cell, 26: 259-67 (1981) 1981 Mostra il sommario Proteolytic fragments of human plasma fibronectin were used to identify monoclonal antibodies reacting with the various domains of fibronectin. One of these antibodies, which reacts with cell-attachment-promoting fragments of fibronectin, inhibits attachment of cells to fibronectin-coated surfaces. A cell-attachment-promoting, chymotryptic, 120 kilodalton fragment was cleaved further with pepsin into three main fragments. The smallest, 15 kilodalton fragment was purified by affinity chromatography on the cell-attachment-inhibiting antibody insolubilized on Sepharose. This fragment is active in promoting cell attachment but lacks the other known binding activities of fibronectin. It can be localized between the collagen-binding and heparin-binding domains, about 127 to 197 kilodaltons from the NH2 terminus of the polypeptide. These results show that the interaction of fibronectin with cells is restricted to a defined portion of the molecule and is independent of the direct involvement of the known affinities toward other macromolecules.	6174240

Panoramica dei riferimenti bibliografici

Codice d'identificazione nel Pub Med

Scheda tecnica

Titolo
PURIFIED HUMAN FIBRONECTIN ALPHA-CHYMOTRYPTIC FRAGMENT 120K

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F1904 Sigma-AldrichFibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified

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