Direct Detect® Spectrometer
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| Life Science Research > Protein Detection and Quantification > Instruments for Protein Analysis > IR-Based Quantitation Instrument |
What if you never had to run another Bradford or BCA, ever? The Direct Detect® system provides more accurate results without the pitfalls of colorimetric assays. By measuring amide bonds in protein chains, the system accurately quantitates an intrinsic component of every protein without relying on amino acid composition, dye binding properties or reduction-oxidation (redox) potential. You can evaluate major components of complex mixtures separable from the Amide I & II region, like lipids and carbohydrates—making lysates and membrane preps easier than ever!
Features & Benefits
- IR spectrometry measures amide bond absorbance for more reproducible quantitation across all proteins and peptides
- Improves accuracy over traditional colorimetric assays
- More compatible with detergents, reducing agents and other buffer components that interfere with traditional protein quantitation assays, providing more information from your sample
- Preserves precious samples—requires only 2 μL per analysis
- New software module can now quantify long aliphatic chains in lipids and detergents
Applications
Protein Quantitation, Lipid Analysis, Peptide Quantitation
Product Performance
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| Quantitation Approach | Assay Range | Sample Volume (tube/plate) |
| Direct Detect® System | 250-5,000 µg/mL | 2 µL |
| Bradford Assay | 20-2,000 µg/mL | 50 µL/25 µL |
| Micro BCA™ Assay | 2-4 µg/mL | 500 µL/150 µL |
| Lowry Assay | 10-1,500 µg/mL | 200 µL/40 µL |