| What do I need to assemble diffusion chambers for studying the effects of implanted cells? | You need three items: (1) Diffusion chamber rings with holes and nylon thread for plugging the holes, catalog number PR00 014 01, (2) 13 mm membrane filters, either 0.22 micron pore size (catalog number GSWP 013 00) or 0.45 micron pore size (catalog number HAWP 013 00), and (3) MF filter cement, catalog number XX70 000 00. Additional nylon thread can be purchased separately under the catalog number PR00 000 00. |
| How do I assemble a diffusion chamber containing tissue for implantation? | - Wet a single membrane filter (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot it to absorbent paper to remove excess water.
- Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry.
- Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
- Moisten the half-completed chamber and the separate sterile filter with a sterile physiological solution, and aseptically place the tissue to be studied in the half-completed chamber assembly.
- Glue the cover filter in place using sterile technique.
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