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2114 Detergent-OUT™ Micro Kit

The Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.

Detergent-OUT™ Micro Kit: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Key Applications: Protein Clean-up
2114
1 kit  10 columns
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Key Applications
PCU
Description
Catalogue Number2114
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionDetergent-OUT™ Micro Kit
OverviewThe Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume.
Materials Required but Not DeliveredCentrifuge

Centrifuge tubes
Product Information
Components
  • Detergent-OUT Columns-DTG-100X 10 Micro
  • Collection Tubes (2 mL) 10
Applications
ApplicationThe Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.
Key Applications
  • Protein Clean-up
Application NotesPROTOCOL:

1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.

5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging Information
Material Size1 kit
Material Package10 columns
Global Trade Item Number
Numero di catalogo GTIN
2114 04053252278426