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69182 DNase I, RNase free

69182
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
69182-3 Bottiglia di vetro 1000 u
Description
Overview

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation. For applications requiring the use of DNase I for random double-stranded cleavage of DNA, try DNase I, ds Qualified.

Catalogue Number69182
Brand Family Novagen®
SynonymsRNase-free DNase I
References
Product Information
Unit of DefinitionOne unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
69182-3 07790788055844

Documentation

DNase I, RNase free MSDS

Titolo

Scheda di sicurezza (MSDS) 

DNase I, RNase free Certificati d'Analisi

TitoloNumero di lotto
69182

Citazioni

Titolo
  • K. M. Hallamaa, G. F. Browning and S. L. Tang. (2006) Lipoprotein multigene families in Mycoplasma pneumoniae. Journal of Bacteriology 188, 5393-5399.
  • G. Ross, et al. (2005) Tuberoinfundibular peptide of 39 residues: a new mediator of cardiac function via nitric oxide production in the rat heart. Endocrinology 146, 2221-2228.