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69725 λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen

λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
69725
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Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
69725-3 Bottiglia di vetro 10 rxn
Description
OverviewThe λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69725
Brand Family Novagen®
References
Product Information
•200 µlλDE3 Phage Lysate
•200 µlHelper Phage Lysate
•200 µlSelection Phage Lysate
•50 µlT7 Tester Phage Lysate
•200 µlHMS174(DE3) glycerol stock
•1 µgpLysS DNA
•1 µgpLysE DNA
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
69725-3 04055977258981

Documentation

λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen MSDS

Titolo

Scheda di sicurezza (MSDS) 

λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen Certificati d'Analisi

TitoloNumero di lotto
69725

Citazioni

Titolo
  • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
  • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
  • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
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    Protocolli per l'uso

    Titolo
    TB053 Academic and Non-profit Laboratory Assurance Letter

    Mappa di un vettore

    Titolo
    TB107 pLysS and pLysE Plasmid Map