251520 Sigma-AldrichDAF-FM DA - CAS 254109-22-3 - Calbiochem
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
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Sinonimi: 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
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Panoramica
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Tabella delle specifiche principali
| CAS # | Empirical Formula |
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| 254109-22-3 | C₂₅H₁₈F₂N₂O₇ |
Products
| Numero di catalogo | Confezionamento | Qtà/conf | |
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| 251520-1MG | Bottiglia di vetro | 1 mg |
| Description | |
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| Overview | A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8. |
| Catalogue Number | 251520 |
| Brand Family | Calbiochem® |
| Synonyms | 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate |
| Product Information | |
|---|---|
| CAS number | 254109-22-3 |
| ATP Competitive | N |
| Declaration | Manufactured by Daiichi Pure Chemicals Co., Ltd |
| Form | Pale yellow solution |
| Formulation | A 5 mM solution in DMSO. |
| Hill Formula | C₂₅H₁₈F₂N₂O₇ |
| Chemical formula | C₂₅H₁₈F₂N₂O₇ |
| Reversible | N |
| Structure formula Image | |
| Quality Level | MQ100 |
| Applications |
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| Biological Information | |
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| Primary Target | Nitric oxide (NO) fluorescent indicator |
| Purity | ≥98% by HPLC |
| Physicochemical Information | |
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| Cell permeable | Y |
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| Safety Information according to GHS |
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| Safety Information | |
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| R Phrase | R: 36/38 Irritating to eyes and skin. |
| S Phrase | S: 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
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| Specifications |
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| Global Trade Item Number | |
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| Numero di catalogo | GTIN |
| 251520-1MG | 04055977198515 |
Documentation
DAF-FM DA - CAS 254109-22-3 - Calbiochem MSDS
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DAF-FM DA - CAS 254109-22-3 - Calbiochem Certificati d'Analisi
| Titolo | Numero di lotto |
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| 251520 |
Riferimenti bibliografici
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| Kojima, H., et al. 2001. J. Neurochem. 76, 1404. Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876. Itoh, Y., et al. 2000. Anal. Biochem. 287, 203. Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209. |