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HTS056M ChemiScreen™ Membrane Preparation
Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)

Human FPRL1 / ALX / HM63 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

ChemiScreen™ Membrane Preparation
Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Host Cell: Chem-1 Uni Prot Number: P25090 Entrez Gene Number: NM_001005738.1, NM_001462.3
HTS056M
1 mL  200 units
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

SpeciesHost CellsProtein Target
Human Chem-1FPRL1 / ALX / HM63
Description
Catalogue NumberHTS056M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiScreen™ Membrane Preparation
Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1)
OverviewHuman FPRL1
Background InformationFPRL1 (formyl peptide receptor-like 1, also known as FPR2) is a GPCR that belongs to the N-formyl peptide receptor family. Initially described as a receptor for lipoxin A4, FPRL1 has been shown to bind to a synthetic peptide WKYMVm, amyloid beta peptides, and N-formylated mitochondrial peptides and mediates phagocyte chemotaxis (Fiore et al., 1994; Le et al., 1999; Rabiet et al., 2005; Iribarren et al., 2005). Chemicon's FPRL1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of FPRL1 interactions with its ligands. The membrane preparations exhibit a Kd of 1 nM for WKYMVm in a GTPγS binding assay.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to 0.5 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80°C.
Quality LevelMQ100
Applications
ApplicationHuman FPRL1 / ALX / HM63 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay and GTPγS binding.
Biological Information
Concentration0.5 mg/mL
Entrez Gene Number
Gene Symbol
  • FPRL1
  • RFP
  • FPR2A
  • FMLPX
  • FPR2
  • HM63
  • FPRH2
  • FMLP-R-I
  • LXA4R
  • FPRH1
  • ALXR
  • FMLP-R-II
GPCR ClassA
Host CellsChem-1
Protein TargetFPRL1 / ALX / HM63
Target Sub-FamilyN-formylpeptide
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P25090 # Low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
SIZE: 351 amino acids; 38964 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
SIMILARITY: SwissProt: P25090 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled WKYMVm is added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) pre-wetted with water, and washed 2 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4. The plate is dried and counted.

One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific WKYMVm stimulated [35S]-GTPγS binding when final concentration of 0.3nM [35S]-GTPγS was used.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceKd for WKYMVm: 0.6 nM in a GTPγS binding assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size1 mL
Material Package200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
HTS056M 04053252368479

Documentation

ChemiScreen™ Membrane Preparation
Recombinant Human FPRL1 (Formyl Peptide Receptor-Like 1) Certificati d'Analisi

TitoloNumero di lotto
ChemiScreenTM MEMBRANE PREPARATION RECOMBINANT HUMAN FPRL1 N-FORMYL PEPTIDE FAMILY RECEPTOR - 2383398 2383398

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Role of formyl peptide receptor-like 1 (FPRL1/FPR2) in mononuclear phagocyte responses in Alzheimer disease
Iribarren, P. et al.
Immunol. Res., 31(3):165-176 (2005)  2004

15888909 15888909
Human mitochondria-derived N-formylated peptides are novel agonists equally active on FPR and FPRL1, while Listeria monocytogenes-derived peptides preferentially activate FPR
Rabiet, M. J. et al.
Eur. J. Immunol., 35(8):2486-2495 (2005)  2004

16025565 16025565
Utilization of two seven-transmembrane, G protein-coupled receptors, formyl peptide receptor-like 1 and formyl peptide receptor, by the synthetic hexapeptide WKYMVm for human phagocyte activation.
Le, Y, et al.
J. Immunol., 163: 6777-84 (1999)  1998

Mostra il sommario 		10586077 10586077
Identification of a human cDNA encoding a functional high affinity lipoxin A4 receptor
Fiore, S. et al.
J. Exp. Med., 180(1):253-260 (1994)  1993

8006586 8006586

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Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations