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14-476 Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
14-476
10 µg  Also available in 250μg size, please inquire for pricing and availability.
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Replacement Information
Description
Catalogue Number14-476
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg
OverviewComplex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceAll are expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.
Gene Symbol
  • CDK7
  • p39MO15
  • CAK
  • CAK1
  • MO15
  • CDKN7
  • STK1
Protein TargetCDK7/cyclinH/MAT1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P50613 # Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
SIZE: 346 amino acids; 39038 Da
SUBUNIT: Associates primarily with cyclin H and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Interacts with PUF60.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Phosphorylation of Ser-164 during mitosis inactivates the enzyme. & Phosphorylation of Thr-170 is required for activity.
SIMILARITY: SwissProt: P50613 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight39.9kDa (cdk7), 37.8kDa (cyclin H), 62.8kDa (MAT1).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of cdk7 substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size, please inquire for pricing and availability.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
14-476 04053252373541

Documentation

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg MSDS

Titolo

Scheda di sicurezza (MSDS) 

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg Certificati d'Analisi

TitoloNumero di lotto
Cdk7/cyclinH/MAT1, active - 1906800-B 1906800-B
Cdk7/cyclinH/MAT1, active - D8HN020U-F D8HN020U-F
Cdk7/cyclinH/MAT1, active - D8HN020U-G D8HN020U-G
Cdk7/cyclinH/MAT1, active -WAB0365-B WAB0365-B

FAQ

DomandaRisposta
How is this complex produced?It is a coexpressed protein - the cells are hit with all three viruses and the complex is purified via the his-tagged CDK - there is no invitro reconstitution. We are not able to comment if there are higher-order dimers (one complex dimerising with another).
What type of buffer is best for lysis when attempting to preserve a protein?The best buffer is Buffer A. It is composed of 50mM Tris/HCl, pH 7.5, 1mM EGTA, 1mM EDTA, 1mM Sodium Orthovanadate (activated), 10mM Na B-glycerolphosphate, 50mM NaF, 5mM Na pyrophosphate, 1% w/v Triton X-100, 0.1% w/v 2-mercaptoethanol, 1ug/ml each of aprotinin, leupeptin and pepstatin, 1mM PMSF and 1mM microcystin (this is optional). QS with water. You may store Buffer A in aliquots at -20°C for 6 months.

Prodotti e applicazioni correlate

Alternative Packsize

Numero di catalogo Descrizione
14-476M Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 250 µg

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Categorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases