Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

03-189 CBP (catalytic domain) human recombinant

03-189
100 µg  
Purchase on Sigma-Aldrich

Panoramica

Tabella delle specifiche principali

Key ApplicationsSpeciesUni Prot Number
Enzyme AssaysHuman Q92793
Description
Catalogue Number03-189
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCBP (catalytic domain) human recombinant
OverviewCBP (catalytic domain) human recombinant expressed in E. Coli
Background InformationAlthough originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
Product Information
FormatPurified
PresentationIn liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationCBP (catalytic domain) human recombinant protein expressed in E. Coli.
Key Applications
  • Enzyme Assays
Application NotesAcetyltransferase assays
Biological Information
PurityPurity >65% by SDS-PAGE
SourceCatalytic domain (residues 1319-1710) of human CBP histone acetyltransferase
Specific Activity[Varies with lot. For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.] XXX 250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).
UniProt Number
Molecular Weight~48 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.
Packaging Information
Material Size100 µg
Global Trade Item Number
Numero di catalogo GTIN
03-189 04053252550836