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70794 BugBuster® GST•Bind™ Purification Kit

70794
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
70794-3 Bottiglia di vetro 1 kit
Description
Overview

The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.



Catalogue Number70794
Brand Family Novagen®
References
Product Information
2 x 100 mlBugBuster Protein Extraction Reagent
10,000 UBenzonase Nuclease, purity > 90%
10 mlGST•Bind Resin
pkg/4Chromatography Columns
2 x 100 ml10X GST Bind/Wash Buffer
40 ml10X Glutathione Reconstitution Buffer
1 gGlutathione, Reduced
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
70794-3 04055977273236

Documentation

BugBuster® GST•Bind™ Purification Kit MSDS

Titolo

Scheda di sicurezza (MSDS) 

BugBuster® GST•Bind™ Purification Kit Certificati d'Analisi

TitoloNumero di lotto
70794

Citazioni

Titolo
  • Xiaobing Chen, et al. (2008) Organization of the core structure of the postsynaptic density. Procedings of the National Academy of Science 105, 4453-4458.
  • Bryan T. Carroll, et al. (2006) Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. Journal of Biological Chemistry 281, 35989-35996.
  • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
  • Protocolli per l'uso

    Titolo
    TB235 GST•Bind™ Kits
    TB245 BugBuster® Protein Extraction Reagent