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Clicchi OK per chiudere MILLIPLEX® MAP, oppure Annulla per tornare alla selezione.
Scelga pannelli su misura e kit premiscelati, OPPURE le microsfere MAPmates™ per la trasduzione del segnale
Progetti e calcoli il prezzo dei Suoi kit MILLIPLEX® MAP.
Pannelli su misura e kit premiscelati
La nostra ampia gamma comprende pannelli multiplex che consentono di scegliere, nell'ambito del pannello, gli analiti più adatti per le Sue esigenze. Oppure Lei può scegliere le citochine premiscelate o i kit single plex.
Kit e MAPmates™ per studi di trasduzione del segnale
Scelga i kit premiscelati che permettono di esplorare interi processi e pathway. Oppure progetti kit su misura scegliendo le microsfere MAPmates™ single plex e seguendo le linee guida fornite.
Le seguenti MAPmates™ non possono essere dosate insieme: -MAPmates™ che richiedono tamponi differenti per l'analisi. -MAPmate™ fosfo-specifiche e totali, es GSK3β totale e GSK3β (Ser 9). -MAPmates™ PanTyr e sito-specifiche come fosfo-recettore EGF e fosfo-STAT1 (Tyr701). -Più di 1 fosfo-MAPmate™ per un solo bersaglio (Akt, STAT3). -GAPDH e β-Tubulina non possono essere miscelati con kit o MAPmates™ contenenti panTyr.
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Elenca
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Selezioni una specie, un tipo di pannello, un kit o un tipo di campione
Per cominciare a progettare il Suo kit MILLIPLEX® MAP, selezioni una specie, un tipo di pannello o un kit di Suo interesse.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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List
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Specie
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96-Well Plate
Qtà
Numero di catalogo
Descrizione dell'ordine
Qtà/conf
Prezzo di listino
Aggiungi altri reagenti (Le microsfere MAPmate devono essere utilizzate con un tampone ed un kit di rilevazione)
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Descrizione dell'ordine
Qtà/conf
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Opzione salva-spazio I Clienti che ordinano diversi kit possono scegliere di ridurre lo spazio necessario per lo stoccaggio, rinunciando al confezionamento del kit e ricevendo i vari reagenti per il saggio multiplex in buste di plastica.
Questo articolo è stato aggiunto ai prodotti preferiti.
Il prodotto è stato aggiunto al carrello.
Ora può disegnare un altro kit su misura, scegliere un kit premiscelato, procedere con il check out dell'ordine, oppure chiudere lo strumento per gli ordini on-line.
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più
BCIP/NBT: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
It is not recommended for immunohistochemistry or in situ hybridization. Functionality check performed on final product by dot blot. Additional QC testing is preformed on a lot-specific basis: pH: 9.7-9.9 Stability at 4°C Stability at 18-26°C
Suggested procedure for immunoblot staining with BCIP/NBT:
1. Complete all required incubations with antibodies and alkaline-phosphatase-labeled probes. 2. After the final binding reaction with the alkaline-phosphatase labeled probe, wash the membrane thoroughly in a buffer such as Tris-buffered saline (TBS) containing 0.1% Tween®-20 detergent. DO NOT USE PHOSPHATE BUFFERS; inorganic phosphate is a potent inhibitor of alkaline phosphatase. 3. Following the final wash, completely cover the membrane with BCIP/NBT Solution and incubate, protected from light, for 5-15 min, or until the desired color intensity is obtained (dark purple bands or dots will appear at the sites of enzyme activity). Note: Variables associated with assay conditions will dictate the proper reaction time. If the color develops almost immediately, the NBT-formazan deposit may flake off the membrane. If this occurs, further dilution of the alkaline phosphatase probe is recommended. A fine line of formazan deposit circumscribing the band or dot, with no deposit in the center, also suggests that further dilution of the alkaline phosphatase probe is needed. 4. Stop the reaction by washing the membrane thoroughly in distilled water. Note: There should be little or no background. Presence of excessive background staining indicates incomplete removal of unbound alkaline phosphatase from the membrane. To remedy this problem, increase the number of wash steps or washing times. 5. Air-dry membranes and store at room temperature, protected from light.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Special Instructions
Discard if the solution turns purple or turbid. The reagent is stable at room temperature, but we recommend storage in the refrigerator to increase the shelf life. Warm to assay temperature before use.
De Jong, A.S., et al. 1985. Histochem. J.17, 1119.
Scheda tecnica
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
BCIP/NBT provided as a stable single component, ready-to-use substrate, sterile-filtered through a 0.22 µm filter. Specifically designed for immunoblotting techniques. BCIP/NBT is the substrate of choice for alkaline phosphatase-based immunoblotting procedures. Hydrolysis of the phosphate ester of BCIP by alkaline phosphatase yields powerful reducing compounds that react rapidly with NBT and convert it to the insoluble NBT-formazan. This reaction product is readily visible on the blotting membrane as dark purple deposits that do not fade upon drying.
Background
BCIP/NBT is the substrate of choice for alkaline phosphatase-based immunoblotting procedures. Hydrolysis of the phosphate ester of BCIP by alkaline phosphatase yields powerful reducing compounds that react rapidly with NBT and convert it to the insoluble NBT-formazan. This reaction product is readily visible on the blotting membrane as dark purple deposits that do not fade upon drying.
Form
Clear to pale yellow liquid
Formulation
0.577 mM BCIP, 0.122 mM NBT in a proprietary solution.
Recommended reaction conditions
Suggested procedure for immunoblot staining with BCIP/NBT:
1. Complete all required incubations with antibodies and alkaline-phosphatase-labeled probes.
2. After the final binding reaction with the alkaline-phosphatase labeled probe, wash the membrane thoroughly in a buffer such as Tris-buffered saline (TBS) containing 0.1% Tween®-20 detergent. DO NOT USE PHOSPHATE BUFFERS; inorganic phosphate is a potent inhibitor of alkaline phosphatase.
3. Following the final wash, completely cover the membrane with BCIP/NBT Solution and incubate, protected from light, for 5-15 min, or until the desired color intensity is obtained (dark purple bands or dots will appear at the sites of enzyme activity).
Note: Variables associated with assay conditions will dictate the proper reaction time. If the color develops almost immediately, the NBT-formazan deposit may flake off the membrane. If this occurs, further dilution of the alkaline phosphatase probe is recommended. A fine line of formazan deposit circumscribing the band or dot, with no deposit in the center, also suggests that further dilution of the alkaline phosphatase probe is needed.
4. Stop the reaction by washing the membrane thoroughly in distilled water.
Note: There should be little or no background. Presence of excessive background staining indicates incomplete removal of unbound alkaline phosphatase from the membrane. To remedy this problem, increase the number of wash steps or washing times.
5. Air-dry membranes and store at room temperature, protected from light.
Preservative
None
Comments
It is not recommended for immunohistochemistry or in situ hybridization. Functionality check performed on final product by dot blot. Additional QC testing is preformed on a lot-specific basis: pH: 9.7-9.9 Stability at 4°C Stability at 18-26°C
Suggested procedure for immunoblot staining with BCIP/NBT:
1. Complete all required incubations with antibodies and alkaline-phosphatase-labeled probes. 2. After the final binding reaction with the alkaline-phosphatase labeled probe, wash the membrane thoroughly in a buffer such as Tris-buffered saline (TBS) containing 0.1% Tween®-20 detergent. DO NOT USE PHOSPHATE BUFFERS; inorganic phosphate is a potent inhibitor of alkaline phosphatase. 3. Following the final wash, completely cover the membrane with BCIP/NBT Solution and incubate, protected from light, for 5-15 min, or until the desired color intensity is obtained (dark purple bands or dots will appear at the sites of enzyme activity). Note: Variables associated with assay conditions will dictate the proper reaction time. If the color develops almost immediately, the NBT-formazan deposit may flake off the membrane. If this occurs, further dilution of the alkaline phosphatase probe is recommended. A fine line of formazan deposit circumscribing the band or dot, with no deposit in the center, also suggests that further dilution of the alkaline phosphatase probe is needed. 4. Stop the reaction by washing the membrane thoroughly in distilled water. Note: There should be little or no background. Presence of excessive background staining indicates incomplete removal of unbound alkaline phosphatase from the membrane. To remedy this problem, increase the number of wash steps or washing times. 5. Air-dry membranes and store at room temperature, protected from light.
Storage
Protect from light
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Discard if the solution turns purple or turbid. The reagent is stable at room temperature, but we recommend storage in the refrigerator to increase the shelf life. Warm to assay temperature before use.
Toxicity
Standard Handling
References
De Jong, A.S., et al. 1985. Histochem. J.17, 1119.
