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04-293 Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors, Kinases & Phosphatases Gene Symbol: ERBB2, HER2, HER-2, NGL, herstatin, erbB-2, C-erbB-2, NEU, TKR1, p185erbB2, HER-2/neu UniProt Number: P04626
04-293
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, MELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-293
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-erbB2 (Thr686) Antibody, clone 7F8
Alternate Names
  • NEU
  • NGL
  • HER2
  • TKR1
  • HER-2
  • c-erb B2
References
Product Information
FormatPurified
Control
  • Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
PresentationIn 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2
Clone7F8
HostMouse
SpecificityRecognizes phosphorylated erbB2 on Threonine 686
IsotypeIgG1
Species Reactivity
  • Canine
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Gene Symbol
  • ERBB2
  • HER2
  • HER-2
  • NGL
  • herstatin
  • erbB-2
  • C-erbB-2
  • NEU
  • TKR1
  • p185erbB2
  • HER-2/neu
Modifications
  • Phosphorylation
Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight180 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
04-293 04053252472848

Documentation

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 Certificati d'Analisi

TitoloNumero di lotto
Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) 3013622
Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) 2988851
Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) 2910369
Anti-phospho-erbB2 (Thr686), clone 7F8 (Mouse monoclonal IgG1) - 2061581 2061581
Anti-phospho-erbB2 (Thr686), clone 7F8 - DAM1485118 DAM1485118
Anti-phospho-erbB2 (Thr686), clone 7F8 - DAM1794016 DAM1794016
Anti-phospho-erbB2 (Thr686), clone 7F8 - DAM1805278 DAM1805278
Anti-phospho-erbB2 (Thr686), clone 7F8 -2638054 2638054
Anti-phospho-erbB2 (Thr686), clone 7F8 -2707856 2707856

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
The duration of phorbol-inducible ErbB2 tyrosine dephosphorylation parallels that of receptor endocytosis rather than threonine-686 phosphorylation: implications for the physiological role of protein kinase C in growth factor receptor signalling
Ouyang, X, et al
Carcinogenesis, 19:2013-9 (1998)  1998

9855018 9855018
Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
Ouyang, X, et al
J Biol Chem, 271:21786-92 (1996)  1996

8702975 8702975

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Categorie

Life Science Research > Antibodies and Assays > Primary Antibodies