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07-357 Anti-phospho-eNOS/NOS III (Ser114) Antibody

Detect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.

Anti-phospho-eNOS/NOS III (Ser114) Antibody: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Research Sub-Category: Oxidative Stress Gene Symbol: NOS3, cNOS, ECNOS, eNOS, EC-NOS, NOSIII UniProt Number: P29474
07-357
100 µL  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-357
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-eNOS/NOS III (Ser114) Antibody
OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Product Information
FormatAffinity Purified
Presentation0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%
Quality LevelMQ100
Applications
ApplicationDetect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.
HostRabbit
SpecificityeNOS/NOS III phosphorylated on serine 114
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
  • cNOS
  • ECNOS
  • eNOS
  • EC-NOS
  • NOSIII
Modifications
  • Phosphorylation
Purification MethodImmunoaffinity Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Molecular Weight132 kDa
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size100 µL
Global Trade Item Number
Numero di catalogo GTIN
07-357 04053252404016