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09-258 Anti-phospho-PAK1 (Ser199/Ser204) Antibody

Detect phospho-PAK1 (Ser199/Ser204) using this Anti-phospho-PAK1 (Ser199/Ser204) Antibody validated for use in WB.

Anti-phospho-PAK1 (Ser199/Ser204) Antibody: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PAK1, MGC130001, Alpha-PAK, MGC130000, PAK-1, PAKalpha, p65-PAK, EC 2.7.11.1, p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), Serine/threonine-protein kinase PAK 1 UniProt Number: Q13153
09-258
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number09-258
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-PAK1 (Ser199/Ser204) Antibody
Background Informationp21-activated kinases (PAK) are Ser/Thr kinases, which are activated by, and are effectors of Rho family of GTPases. As such, they are implicated in Actin polymerization and activation of the stress-activated kinase cascades. PAK1 is phosphorylated by cdk5, resulting in its inhibition. PAK2 can be activated by Caspase-cleavage in addition to p21-binding, implicating it in cytoskeletal changes during apoptosis. PAK3 is expressed at high levels in post-mitotic neurons of the developing post-natal cerebral cortex and hippocampus, and is the mutant gene thought responsible for non-syndromic X-linked mental retardation.
References
Product Information
FormatAffinity Purified
PresentationAffinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol
Quality LevelMQ100
Applications
ApplicationDetect phospho-PAK1 (Ser199/Ser204) using this Anti-phospho-PAK1 (Ser199/Ser204) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenAmino Acids encompassing and including phosphorylated Ser199 and Ser204 of human PAK1. Sequence is highly homologous (greater than 90%) to PAK2 and PAK3.
EpitopeSer199/Ser204 (Central)
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes PAK1 phosphorylated on Ser199 and Ser204 (human)/Ser198 and Ser203 (mouse). Immunogen sequence is higly homolgous (greater than 90%) with PAK2 and PAK3.
Species Reactivity
  • Human
Species Reactivity NoteMouse and Rat expected to react based on 100% homology of immunogen.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryPAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol
  • PAK1
  • MGC130001
  • Alpha-PAK
  • MGC130000
  • PAK-1
  • PAKalpha
  • p65-PAK
  • EC 2.7.11.1
  • p21-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • Serine/threonine-protein kinase PAK 1
Modifications
  • Phosphorylation
Purification MethodDouble affinity purification over both non-phospho-peptide column followed by phopshorylated peptide column.
UniProt Number
UniProt SummaryFUNCTION: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
SIZE: 545 amino acids; 60647 Da
ENZYME REGULATION: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-423 and allows the kinase domain to adopt an active structure. Also activated by binding to GTP-bound CDC42, independent of the phosphorylation state of Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation
SUBUNIT: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. Interacts with ARHGEF7. Also interacts with CRIPAK.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation.
PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.
SIMILARITY: SwissProt: Q13153 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
Molecular Weight65 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on untreated and pervanadate-treated Jurkat cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
09-258 04053252363498

Documentation

Anti-phospho-PAK1 (Ser199/Ser204) Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-phospho-PAK1 (Ser199/Ser204) Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-phospho-PAK1 (Ser199/Ser204) - 2383536 2383536
Anti-phospho-PAK1 (Ser199/Ser204) - 2433448 2433448
Anti-phospho-PAK1 (Ser199/Ser204) - 1984816 1984816
Anti-phospho-PAK1 (Ser199/Ser204) - 3457124 3457124
Anti-phospho-PAK1 (Ser199/Ser204) - 3690680 3690680
Anti-phospho-PAK1 (Ser199/Ser204) - 3792962 3792962
Anti-phospho-PAK1 (Ser199/Ser204) - 4047614 4047614
Anti-phospho-PAK1 (Ser199/Ser204) - 4152468 4152468
Anti-phospho-PAK1 (Ser199/Ser204) - NG1523245 NG1523245
Anti-phospho-PAK1 (Ser199/Ser204) -2716284 2716284
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Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Magnetic nanoparticle-mediated massively parallel mechanical modulation of single-cell behavior.
Tseng, P; Judy, JW; Di Carlo, D
Nature methods  9  1113-9  2011

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