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ABT282 Anti-phospho-NUMA1 Antibody (Thr2055)

This Anti-phospho-NUMA1 Antibody (Thr2055) is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho-NUMA1 (Thr2055).

Anti-phospho-NUMA1 Antibody (Thr2055): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: NUMA1, NUMA UniProt Number: Q14980
ABT282
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT282
DescriptionAnti-phospho-NUMA1 Antibody (Thr2055)
Alternate Names
  • Nuclear mitotic apparatus protein 1
  • NuMA protein
  • SP-H antigen
Background InformationNUMA1 or NUMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NUMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NUMA1 resides in the nuclear matrix during interphase. NUMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NUMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis. NUMA1 phosphorylation occurs by CDK1 and phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-NUMA1 Antibody (Thr2055) is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho-NUMA1 (Thr2055).
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot blocked NUMA1 phosphorylated at Thr2055 in Hydroxyurea treated HeLa nuclear extract.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the tail region of human NUMA1 phosphorylated at Thr2055.
EpitopeTail Region
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NUMA1
  • NUMA
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~230 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NUMA1 phosphorylated at Thr2055 in 10 µg of HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABT282 04055977039481