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04-746 Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

04-746
100 µL  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

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Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DB, Mplex, PIARbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-746
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal
Alternate Names
  • H3T3P
  • Histone H3 (phospho T3)
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
References
Product Information
FormatCulture Supernatant
Control
  • Acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid
PresentationCultured supernantant in 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325 (Rabbit Monoclonal Antibody) validated in WB, DB, Mplex, PIA to detect phospho-Histone H3 (Thr3) also known as H3T3P, Histone H3 (phospho T3).
Key Applications
  • Western Blotting
  • Dot Blot
  • Multiplexing
  • Peptide Inhibition Assay
Application NotesWB, ChIP, BD, PIA
Biological Information
ImmunogenPeptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
CloneJY325
HostRabbit
SpecificityHistone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol
  • HIST3H3
  • H3FT
  • MGC126886
  • H3t
  • MGC126888
  • H3.4
  • H3T
  • H3/g
  • H3/t
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
Molecular Weight17 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid, but not unmodified recombinant histone H3
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
04-746 04053252284540

Documentation

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal Certificati d'Analisi

TitoloNumero di lotto
Anti-phospho-Histone H3 (Thr3), -2820740 2820740
Anti-phospho-Histone H3 (Thr3), clone JY325 2474938
Anti-phospho-Histone H3 (Thr3), clone JY325 - 3243553 3243553
Anti-phospho-Histone H3 (Thr3), clone JY325 - 3519310 3519310
Anti-phospho-Histone H3 (Thr3), clone JY325 - 3697988 3697988
Anti-phospho-Histone H3 (Thr3), clone JY325 - 3792005 3792005
Anti-phospho-Histone H3 (Thr3), clone JY325 - DAM1498961 DAM1498961

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiApplicazioneCodice d'identificazione nel Pub Med
Antitumor activity of a small-molecule inhibitor of the histone kinase Haspin.
Huertas, D; Soler, M; Moreto, J; Villanueva, A; Martinez, A; Vidal, A; Charlton, M; Moffat, D; Patel, S; McDermott, J; Owen, J; Brotherton, D; Krige, D; Cuthill, S; Esteller, M
Oncogene  31  1408-18  2011

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Immunofluorescence21804608 21804608