Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

MABN24 Anti-pan-Shank Antibody, clone N23B/49

Detect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P).

Anti-pan-Shank Antibody, clone N23B/49: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: SHANK2, CORTBP1, ProSAP1, CTTNBP1, SPANK-3, SHANK, PROSAP1, CortBP1, Shank2 UniProt Number: Q9QX74
MABN24
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

100% Performance Guaranteed

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN24
DescriptionAnti-pan-Shank Antibody, clone N23B/49
Alternate Names
  • SH3 and multiple ankyrin repeat domains 2
  • SH3 and multiple ankyrin repeat domains protein 2
  • proline-rich synapse associated protein 1
  • cortactin SH3 domain-binding protein
  • Cortactin-binding protein 1
  • Proline-rich synapse-associated protein 1
  • cortactin binding protein 1
  • GKAP/SAPAP interacting protein
Background InformationShank1, Shank2, and Shank3 make up a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank contains multiple domains for protein-protein interaction including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile α motif domain, and a proline-rich region. Alternative splicing in the Shank family may be a mechanism that regulates the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain.
References
Product Information
FormatPurified
Control
  • Rat brain membrane tissue lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: 1:500 dilution from a previous lot detected Shank in rat cerebellum tissue.

Western Blot Analysis: A previous lot of this antibody detected Shank in extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids. Courtesy of James Trimmer, UC Davis/NIH NeuroMab Facility.
Biological Information
ImmunogenRecombinant protein consisting of SH3/PDZ domain of rat Shank2.
CloneN23B/49
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SHANK2
  • CORTBP1
  • ProSAP1
  • CTTNBP1
  • SPANK-3
  • SHANK
  • PROSAP1
  • CortBP1
  • Shank2
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

SUBUNIT STRUCTURE: Interacts with DNM2 and BAIAP2. Interacts with GRID2 (By similarity). Interacts with CCTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with SLC9A3 and PLCB3. Interacts with DBNL. Interacts with CFTR.

SUBCELLULAR LOCATION: Cytoplasm. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell projection › growth cone. Note: Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons.

TISSUE SPECIFICTY: All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).

DEVELOPMENTAL STAGE: Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.

DOMAIN: The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.

SEQUENCE SIMILARIITIES: Belongs to the SHANK family.

Contains 1 PDZ (DHR) domain.

Contains 1 SAM (sterile alpha motif) domain.

Contains 1 SH3 domain.
Molecular Weight~160 kDa observed. Other isoforms may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Shank on 10 µg of rat brain membrane tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABN24 04053252523830

Documentation

Anti-pan-Shank Antibody, clone N23B/49 MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-pan-Shank Antibody, clone N23B/49 Certificati d'Analisi

TitoloNumero di lotto
Anti-pan-Shank, clone N23B/49 - 2445048 2445048
Anti-pan-Shank, clone N23B/49 - 2043216 2043216
Anti-pan-Shank, clone N23B/49 - 2192072 2192072
Anti-pan-Shank, clone N23B/49 - 2283569 2283569
Anti-pan-Shank, clone N23B/49 - 3328966 3328966
Anti-pan-Shank, clone N23B/49 - 3468322 3468322
Anti-pan-Shank, clone N23B/49 - 3574547 3574547
Anti-pan-Shank, clone N23B/49 - 3886038 3886038
Anti-pan-Shank, clone N23B/49 - 4132012 4132012
Anti-pan-Shank, clone N23B/49 - 4231222 4231222
Show more