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ABN1477 Anti-nSR100 (SRRM4)

Anti-nSR100 (SRRM4), Cat. No. ABN1477, is a highly specific rabbit polyclonal antibody that targets Serine/arginine repetitive matrix protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-nSR100 (SRRM4): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Gene Symbol: SRRM4, KIAA1853 UniProt Number: A7MD48
ABN1477
100 μg  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IH(P)RbPurifiedPolyclonal Antibody
Description
Catalogue NumberABN1477
DescriptionAnti-nSR100 (SRRM4)
Alternate Names
  • Serine/arginine repetitive matrix protein 4
  • Medulloblastoma antigen MU-MB-2.76
  • Neural-specific serine/arginine repetitive splicing factor of 100 kDa
  • Neural-specific SR-related protein of 100 kDa
  • nSR100
Background InformationSerine/arginine repetitive matrix protein 4 (UniProt: A7MD48; also known as Medulloblastoma antigen MU-MB-2.76, Neural-specific serine/arginine repetitive splicing factor of 100 kDa, Neural-specific SR-related protein of 100 kDa, nSR100) is encoded by the SRRM4 (also known as KIAA1853) gene (Gene ID: 84530) in human. nSR100 is a nuclear protein that is specifically expressed in neuronal cells. It serves as a splicing factor that is specifically required for neural cell differentiation. It acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promote neural-specific exon inclusion in many genes that function in neural cell differentiation. It is shown to regulate the splicing of protrudin gene (Zfyve27) transcripts in neuronal cells and promotes splicing of protrudin pre-mRNA so as to include a microexon (exon L) encoding seven amino acids in a neuron-specific manner. The resulting protein known as Protrudin-L promotes neurite outgrowth during neurogenesis. Depletion of nSR100 in Neuro2A cells is shown to impair neurite outgrowth, whereas its ablation in mouse embryos is reported to prevent differentiation of neuronal progenitors in the cortex and impair development of the central and peripheral nervous systems. (Ref.: Ohnishi, T et al. (2017). Sci. Rep. 7: 41130; Quesnel-Vallières, M et al. (2015). Genes Dev. 29(7); 746-759).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-nSR100 (SRRM4), Cat. No. ABN1477, is a highly specific rabbit polyclonal antibody that targets Serine/arginine repetitive matrix protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected nSR100 (SRRM4) in mouse cerebral cortex and human cerebral cortex tissue sections.
Biological Information
ImmunogenGST/His-tagged recombinant frgment corresponding to 104 amino acids from the C-terminal half of human Serine/arginine repetitive matrix protein 4.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Serine/arginine repetitive matrix protein 4 in human and mouse cells. It targets an epitope within 104 amino acids from the C-terminal half.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SRRM4
  • KIAA1853
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~68 kDa observed; 68.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected nSR100 (SRRM4) in 10 µg of human cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABN1477 04054839399718