Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

ABT170 Anti-alpha Tubulin Antibody, nontyrosinated

ABT170
100 µL  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, Porcine, H, RWB, ELISA, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABT170
Replaces04-1117
DescriptionAnti-alpha Tubulin Antibody, nontyrosinated
Alternate Names
  • Tubulin alpha-1A chain
  • Alpha-tubulin 3
  • Tubulin B-alpha-1
  • Tubulin alpha-3 chain
Background InformationMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Product Information
FormatSerum
PresentationRabbit polyclonal serum in buffer containing serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.
Key Applications
  • Western Blotting
  • ELISA
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin and not tyrosinated alpha Tubulin from purified porcine alpha Tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human nontyrosinated alpha Tubulin.
HostRabbit
SpecificityThis antibody detects the nontyrosinated form of alpha Tubulin.
Species Reactivity
  • Mouse
  • Porcine
  • Human
  • Rat
Species Reactivity NoteDemonstrated to react with Mouse and Porcine. Predicted to react with Human and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TUBA1A
  • TUBA3
Purification MethodUnpurified
UniProt Number
Molecular Weight~51 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Numero di catalogo GTIN
ABT170 04053252928123