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MABN633 Anti-a-Synuclein, clone 10D2 Antibody

MABN633
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, ELISAMAffinity PurifiedMonoclonal Antibody
Description
Catalogue NumberMABN633
Replaces04-1053
DescriptionAnti-a-Synuclein, clone 10D2 Antibody
Alternate Names
  • Alpha-synuclein
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • NACP
Background Informationα-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing.
This antibody can be used as a sandwich ELISA pair with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4.
References
Product Information
FormatAffinity Purified
PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-a-Synuclein, clone 10D2 Antibody is an antibody against a-Synuclein for use in western blotting, IHC & ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • ELISA
Application NotesImmunohistochemistry Analysis: A representative lot detected aggregated a-Synuclein in human brain tissues.

ELISA Analysis: A representative lot from an independent laboratory detected aggregated a-Synuclein in a sandwich ELISA with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4 (Kovacs, G. G., et al. (2012). Acta Neuropathol. 123(1):37-50.).
Biological Information
ImmunogenRecombinant protein corresponding to human Synuclein.
Clone10D2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SNCA
  • NACP
  • PARK1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~15 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.5 µg/mL of this antibody detected a-Synuclein in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABN633 04053252938757

Documentation

Anti-a-Synuclein, clone 10D2 Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-a-Synuclein, clone 10D2 Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-a-Synuclein, clone 10D2 - VP2408217
Anti-a-Synuclein, clone 10D2 - QVP1310072 QVP1310072
Anti-a-Synuclein, clone 10D2 - VP1801020 VP1801020
Anti-a-Synuclein, clone 10D2 - VP1902130 VP1902130
Anti-a-Synuclein, clone 10D2 - VP2210121 VP2210121
Anti-a-Synuclein, clone 10D2 - VP2312200 VP2312200
Anti-a-Synuclein, clone 10D2 -VP15100027 VP15100027
Anti-a-Synuclein, clone 10D2 -VP1605073 VP1605073
Anti-a-Synuclein, clone 10D2 Monoclonal Antibody VP1702255

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
An antibody with high reactivity for disease-associated α-synuclein reveals extensive brain pathology.
Kovacs, Gabor G, et al.
Acta Neuropathol., 124: 37-50 (2012)  2011

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Life Science Research > Antibodies and Assays > Primary Antibodies