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MAB5280X Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15, Alexa Fluor® 488

MAB5280X
100 µg  
Purchase on Sigma-Aldrich

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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, R, QuIHCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB5280X
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tyrosine Hydroxylase Antibody, clone 2/40/15, Alexa Fluor® 488
Product Information
FormatAlexaFluor®488
PresentationPurified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Tyrosine Hydroxylase Antibody, clone 2/40/15, Alexa Fluor 488 is an antibody against Tyrosine Hydroxylase for use in IH.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).
Cloneclone 2/40/15
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityTyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Chicken
  • Rat
  • Quail
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Gene Symbol
  • TH
  • TYH
  • EC 1.14.16.2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Product Usage Statements
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots in the dark for up to 6 months after date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Numero di catalogo GTIN
MAB5280X 04053252318412