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ST1070 Anti-Tiam1 Rabbit pAb

This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.

Anti-Tiam1 Rabbit pAb: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: Anti-T-Cell Lymphoma Invasion and Metastasis 1

ST1070
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
ST1070-50UG Fiala di plastica 50 μg
Description
OverviewRecognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
Catalogue NumberST1070
Brand Family Calbiochem®
SynonymsAnti-T-Cell Lymphoma Invasion and Metastasis 1
Application Data
Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
References
ReferencesMertens, A.E., et al. 2003. FEBS Lett. 546, 11.
Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.
Product Information
FormLiquid
FormulationIn Tris-Citrate/phosphate, pH 7.0-8.0.
Positive controlJurkat or NIH3T3 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ST1070-50UG 04055977224405

Documentation

Anti-Tiam1 Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Tiam1 Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
ST1070

Riferimenti bibliografici

Panoramica delle referenze
Mertens, A.E., et al. 2003. FEBS Lett. 546, 11.
Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-October-2007 RFH
SynonymsAnti-T-Cell Lymphoma Invasion and Metastasis 1
ApplicationImmunoblotting (1:1000)
Application Data
Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
BackgroundTiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
IsotypeIgG
Specieshuman, mouse
Positive controlJurkat or NIH3T3 cells
FormLiquid
FormulationIn Tris-Citrate/phosphate, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Highly Toxic
ReferencesMertens, A.E., et al. 2003. FEBS Lett. 546, 11.
Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.