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IM32 Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1)

IM32
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Panoramica

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
B, H, RMMonoclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
IM32-100UG Fiala di plastica 100 μg
Description
OverviewRecognizes the ~28.5 kDa TIMP-1 protein. Also recognizes MMP/TIMP-1 complexes.
Catalogue NumberIM32
Brand Family Calbiochem®
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
Application Data
Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesFujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Positive controlrTIMP-1 Protein (Cat. Nos. PF019 or PF020)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml, see application references)
Immunocytochemistry (see application references)
Paraffin Sections (25 µg/ml, see application references, see comments)
Application CommentsAlso recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified, bovine dental pulp TIMP-1
ImmunogenBovine
Clone7-6C1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Numero di catalogo GTIN
IM32-100UG 07790788053765