Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

MABE1132 Anti-TET2 Antibody, clone 9F7

Anti-TET2 Antibody, clone 9F7 is an antibody against TET2 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.

Anti-TET2 Antibody, clone 9F7 : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: Tet2, Kiaa1546 UniProt Number: Q4JK59
MABE1132
100 μg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

100% Performance Guaranteed

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
MICC, WB, IP, ELISARPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1132
DescriptionAnti-TET2 Antibody, clone 9F7
Alternate Names
  • Methylcytosine dioxygenase TET2
  • Protein Ayu17-449
Background InformationMethylcytosine dioxygenase TET2 (EC 1.14.11.n2; UniProt Q4JK59; also known as Protein Ayu17-449) is encoded by the Tet2 (also known as Kiaa1546) gene (Gene ID 214133) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TET2 Antibody, clone 9F7 is an antibody against TET2 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunoprecipitation
  • ELISA
Application NotesWestern Blotting Analysis: A representative lot detected exogenously expressed TET2, but not TET1 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET2 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 9F7 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET2 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Biological Information
ImmunogenHis-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET2.
EpitopeRegion between DNA-binding and 2-oxoglutarate-binding domains.
Clone9F7
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 9F7 detected exogenously expressed TET2, but not TET1 or TET3, GST fusion (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812). Epitope region is present in all three murine TET2 spliced isoforms reported by UniProt (Q4JK59).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tet2
  • Kiaa1546
Purification MethodProtein G purified.
UniProt Number
Molecular Weight212.1 kDa (isoform 1), 68.06 kDa (isoform 2), 64.50 kDa (isoform 3) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in mouse mouse ESCs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected TET2 in mouse embryonic stem cells (ESCs).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABE1132 04055977350920