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MABN627 Anti-SDHB Antibody, clone 10C5.1

Anti-SDHB Antibody, clone 10C5.1 is an antibody against SDHB for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-SDHB Antibody, clone 10C5.1 : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: SDHB, SDH, SDH1 UniProt Number: P21912
MABN627
100 μg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN627
DescriptionAnti-SDHB Antibody, clone 10C5.1
Alternate Names
  • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
  • SDHB
  • Iron-sulfur subunit of complex II
  • Ip
Background InformationSuccinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1; UniProt P21912; also known as Ip, Iron-sulfur subunit of complex II) is encoded by the SDHB (also known as IP, SDH, CWS2, PGL4, SDH1, SDHIP) gene (Gene ID 6390) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential cancer markers and targets for treatment. Succinate dehydrogenase (SDH), also known as mitochondrial respiratory Complex II, is a holoenzyme consisting of four essential subunits, SDHA (a flavoprotein), SDHB (iron-sulfur protein), and two membrane anchor units SDHC and SDHD. SDH assembly requires two factors, SDHAF1 and SDHAF2, while its function is influenced by the deacetylase activity of SIRT3. SDH is the only enzyme complex known to participate in both the TCA cycle and electron transport chain (ETC.). SDH catalyzes the oxidation of succinate in the TCA to form fumarate, a reaction that is coupled to the reduction of ubiquinone to ubiquinol in the ETC. SDH loss-of-function mutations have been linked to pheochromocytoma (PCC), paraganglioma (PGL), gastrointestinal stromal tumor (GIST), renal cell carcinoma, and ovarian cancer.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SDHB Antibody, clone 10C5.1 is an antibody against SDHB for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected SDHB in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SDHB in human heart, human small intestine, and mouse kidney tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminal half of human SDHB.
EpitopeC-terminal half
Clone10C5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SDHB
  • SDH
  • SDH1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~30 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SDHB in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABN627 04055977300130